Wednesday, 5 February 2014

ANTHOCYANIN RICH TRANSGENIC POTATO!


Pic Courtesy: Solanaceae Co-ordinated Agri Project
Scientists from Nanjing Agricultural University of China have cloned and characterized a potato StAN11 gene involved in anthocyanin biosynthesis regulation. They cloned StAN11, a WD40-repeat gene from potato cultivar Chieftain (Solanum tuberosum L.). StAN11 (HQ599506) contained no intron and its open reading frame (ORF) was 1,029 bp long, encoding a putative protein of 342 amino acids. To verify its role in anthocyanin biosynthesis, StAN11 was inserted behind the CaMV-35S promoter of pCMBIA1304 and the recombination vector was introduced into the potato cultivar Désirée plants by Agrobacterium-mediated transformation.

They observed that the color of transgenic tuber skin was significantly deepened, compared to the wild-type control, which was highly consistent with the accumulation of anthocyanin and expression of StAN11 in transgenic lines tuber skin. 
They also analysed the expression of Flavonone-3-hydroxylase (F3H), Dihydroflavonol reductase (DFR), Anthocyanidin synthase (ANS), and Flavonoid 3-O-glucosyl transferase (3GT) in transgenic plants. Analysis revealed that only DFR was upregulated. 
Their result suggests that StAN11 regulates anthocyanin biosynthesis in potato by controlling DFR expression and accumulation of anthocyanin could be increased through overexpression of StAN11 in the tubers with the genetic background of anthocyanin biosynthesis.
The findings were published online in Journal of Integrative Plant Biology on 4th of February 2014.
Anthocyanins are water soluble pigments that may appear red, purple, or blue depending on the pH. It occur in tissues of higher plants. Recent invitro studies of purified anthocyanin have confirmed its various uses which includes protection against liver injuries; significant reduction of blood pressure; improvement of eyesight; strong anti-inflammatory and antimicrobial activities; inhibition of mutations caused by mutagens from cooked food; and suppression of proliferation of human cancer cells.

Li W, Wang B, Wang M, Chen M, Yin JM, Kaleri GM, Zhang RJ, Zuo TN, You X, Yang Q (2014) Cloning and characterization of a potato StAN11 gene involved in anthocyanin biosynthesis regulation. J Integr Plant Biol 

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